lMx and Serono MAlAclone Assays Compared for Lutropin Determinations in Urine
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چکیده
References 1. Veit J. Untersuchungen Ober den osmotischen Druck zwischen Mutter und Kind. Z Geburtshilfe Gynaekol 1900;42:316-29. 2. Robertson GL. Thirst and vasopressin function in normal and disordered states of water metabolism. J Lab Clin Med 1983;101: 351-71. 3. Robertson GL. Osmoregulation ofthirst and vasopressin secretion: functional properties and their relationship to water balance. In: Schrier RW, ed. Vasopressin. New York: Raven Press, 1985: 203-12. 4. Bevilacqua M, Meroni R, Dagani R, Renesto Norbiato GE, Baruto C, Norbiath G. Role ofblood osmolality in the regulation of vasopressin secretion in men: application of a new radioimmuno-
منابع مشابه
Evaluation of dual-label simultaneous assays for lutropin and follitropin in serum.
We evaluated the analytical performance and clinical utility of three dual-label simultaneous assays for lutropin and follitropin by comparison with widely used individual assays for these analytes (Diagnostic Products Corp.; DPC). Of the three assays evaluated, "Cotropin" (Clinetics Corp.) and "Combostat" (Micromedics Systems, Inc.) compared favorably with the DPC assay with respect to recover...
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In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay involving the BiAb (Bi-EIA) with an immunoenzymatic assay (EIA) in which the s...
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Lutropin was isolated from woman preovulatory urine by immunoaffinity chromatography using a column with monoclonal antibody (mAb) anti-beta hLH(37) coupled to Sepharose CL 4B. As it was demonstrated the isolated hormone, as well as pituitary lutropin, were separated in SDS-PAGE into several well visible fractions with 30-94 kDa molecular mass and scarcely visible fractions with 14-20 kDa. All ...
متن کاملValidation of highly specific and sensitive radioimmunoassays for lutropin, follitropin, and free alpha subunit in unextracted urine.
Measurement of the urinary excretion of lutropin (LH) and follitropin (FSH) and their common free alpha subunit (FAS) assists in monitoring the maturation of the hypothalamic-pituitary-gonadal axis and in understanding the physiology of the pituitary glycoprotein hormones. Here we describe sensitive, specific polyclonal radioimmunoassays for LH and FSH and a monoclonal radioimmunoassay for FAS ...
متن کاملConvenient radioimmunoassay for urinary human choriogonadotropin without interference by urinary human lutropin.
We have devised a radioimmunoassay (RIA) for human choriogonadotropin (hCG) in first morning-voided urine specimens. Concanavalin A, a lectin, is used to extract and concentrate the hCG from urine. A high-affinity antiserum is used, directed to the hCG beta carboxy-terminal peptide, a unique immunological determinant not shared by the beta subunit of human lutropin. This ensures that urinary hu...
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تاریخ انتشار 2005